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Fig. 6. Effect of Myc activation on keratinocyte motility and spreading. Myc over-expressing keratinocytes (Kq-MycER) were compared with keratinocytes expressing the empty retroviral vector (Kq-pBP) or the mutated Myc construct (Kq-106ER). (A) Movements of individual cells plated on collagen are shown. Green spots: start coordinates; red spots: end coordinates. (B) Average speeds of Kq-MycER (n=24), Kq-106ER (n=24) and Kq-pBP (n=23) on collagen. (C) Cell spreading in serum-free medium (FAD-). Cells were stained with phalloidin. Scale bar: 10 µm. (D) Median of cells spreading in serum-free medium (FAD) or medium supplemented with EGF, IGF or cytochalasin D (cyto. D).





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