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Fig. 9. (A) RNA expression profile of downregulated cytoskeleton components and
cytoskeleton regulatory factors in untreated (0d) and treated (1d and 4d)
wild-type and transgenic mice. (B) Northern blot for MyBP-H of RNA from skin
of wild-type (wt) and transgenic (tg) mice, untreated (0d) or treated for 1
day (1d) or 4 days (4d) with OHT. (C) Immunolabelling with antibodies to
adducin and myosin II of skin from wild-type and transgenic animals after 4
days OHT treatment. Scale bar: 10 µm. (D) Cultured human keratinocytes
(Kq-MycER and Kq-pBP) were stained with antibodies to actin (red; left panels)
and myosin II (green; right panels). Cells shown are at the periphery of
clones or at the edges of wounds made in confluent sheets. Scale bar: 5 µm.
Nuclei are stained for Myc using 9E10 antibody. (E-G) Downregulation of
adducin upon activation of Myc visualised by immunofluorescence (E), real-time
PCR (F), and Western blotting (G). (E,G) Adducin protein expression in Myc
expressing human keratinocytes (Kq-MycER) is decreased compared to controls
(Kq-pBP). Scale bar: 10 µm. (F) Relative RNA expression is reduced in skin
of transgenic (tg) animals treated with OHT for 4 days (4d) compared to 4 days
treated wild-type (wt) and untreated (0d) transgenic mice
(P<0.05).
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