Fig. 1. Pigment cell defects in cls/sox10^-/-, nac/mitfa^-/- and cls/sox10^-/-;nac/mitfa^-/- mutant embryos. Lateral views of the dorsal trunk of 3 dpf wild type (A), nac/mitfa^b692/b692 (B), cls/sox10^t3/t3 (C) and cls/sox10 ^t3/t3;nac/mitfa ^b692/b692 (D) embryos. Wild-type embryos have large flat melanophores (black arrow), cls/sox10^-/- and cls/sox10^-/-;nac/mitfa^-/- embryos have a few tiny rounded melanophores (black arrows), but nac/mitfa^-/- embryos lack melanophores. Iridophores (white arrows) are not reduced in nac/mitfa^-/- embryos but are severely reduced in cls/sox10^-/- and cls/sox10^-/-;nac/mitfa^-/-. Double mutant embryos were identified by PCR genotyping.

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