Fig. 6. Sca and Gp150 in endosomes. (A) A single confocal plane. (B-E) Confocal projections of eye discs [merged images in left column, Rab7-GFP (green) and Sca (magenta) in middle and right columns]. (A) Rab7-GFP (green) and Sca (magenta). All large Sca particles are surrounded by Rab7-GFP. Rab7-GFP also labels endosomes lacking Sca protein. (B) HRS (green) and Sca (magenta). Sca particles do not colocalize with HRS, even when the component planes of this confocal projection are examined. (C) GFP-Rab7 (green) and Gp150 (magenta). Most Gp150 is surrounded by GFP-Rab7. (D) Gp150 (green) and Sca (magenta). All large Sca particles colocalize with Gp150. (E) Absence of ß-galactosidase (green) indicates cells homozygous for gp150². Sca protein in magenta. There is less Sca protein in large particle in the absence of Gp150.

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