Fig. 1. vis and achi encode two highly related TGIF-like proteins. (A) Sequence alignment of the predicted VisS and AchiL proteins with other members of the TGIF family. Residues conserved in all family members are highlighted in pink; residues conserved in all but one sequence are highlighted in yellow; residues conserved in a smaller subset of sequences are highlighted in blue; and similarities to Vis/Achi are shown in purple. The alternatively spliced exon in AchiL is indicated by the green arrowheads (also in B). * marks the predicted end of each protein. hTGIF, human TGIF; hTGIF2, human TGIF2; hTGIF2LX, human TGIF2-like on X; mTex1, mouse Tex1; fTGIF, Fugu TGIF; AgTGIF, Anopheles gambiae TGIF. The extent of the homeodomain and three alpha helices (1, 2, 3) is indicated above the sequence by the black and grey bars; the red box marks the three TALE residues in between helices 1 and 2. The residues `AYP', also in the loop between helices 1 and 2, are one of the hallmarks of the TGIF family. (B) Genomic organization of the vis/achi locus. cDNAs predicted to encode visS (SD01238), achiS2 (LD25085) and achiL (LP02076) have been sequenced; transcripts for visL and achiS1 are predicted from the analysis of the genome sequence. The alternatively spliced exons are indicated with an asterisk. Lighter shading indicates non-coding sequences, and the black boxes indicate the homeoboxes in vis and achi. (C) Df(2R)pingpong is a deletion (indicated by the dashed line) of vis, achi and four neighboring genes. The extent of the two rescue P elements for vis (P{vis}) and achi (P{achi}) are indicated.

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