Fig. 6. Expression of cell cycle regulators in pingpong testes. (A,B) Wild-type (A) and pingpong (B) testes stained for DNA (red), CycB (green) and Vis/Achi (blue). In the pingpong mutant CycB is expressed throughout the testes at intermediate levels and is cytoplasmic. Loss of staining is only observed far from the apical tip of the testes where cells are degenerating. The arrows point to the high levels of CycB staining in wild type and more uniform staining in the mutants. (C,D) Wild-type (C) or pingpong (D) testes stained for DNA (red) and CycA (green). In the pingpong mutant CycA is expressed throughout the testes and is primarily cytoplasmic. Loss of staining is only observed at the basal end of the testes where the cells are degenerating. The arrows point to the high levels of CycA staining in wild type and more uniform staining in the mutants. (E,F) Wild-type (E) or pingpong (F) testes stained for DNA (red) and Polo (green). Staining is high at the apical tip in both wild type and the mutant. In the mutant, the levels gradually decrease in cells farther from the apical tip whereas in the wild type, Polo levels are high in older (metaphase) 16-cell cysts.

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