Fig. 1. The expression domains of Pax2 and Pax6 entirely overlap with markers of the early OV progenitor domains. Immunohistochemical analysis of the expression of Pax6 (green, A,E,I) and of Pax2 (red, B,F,J) in serial, 6 µm sections. C, G and K show overlays of the respective Pax2 and Pax6 expression. D, H and L are at higher magnifications. Pax2 and Pax6 expression overlaps in the early OV at E9.5 (A-D) and at E10.0 (E-H). (I-L) At E12.5, Pax2 is restricted mainly to the optic stalk but Pax6 is still expressed in all distal eye components. (M-P) At E9.5, the segregation of RPE markers, such as Mitf (M) and Otx2 (N) is detectable, in contrast to the NR marker Chx10 (O). The adjacent section (P), stained for Pax2, reveals the overlap of Pax2 and Pax6 (see also A and B) with both RPE and NR markers. le, lens; nr, neural retina; os, optic stalk; ov, optic vesicle; rpe, retinal pigmented epithelium; se, surface ectoderm.

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