Fig. 1. eyg loss-of-function mutant phenotype. (A-D) Scanning electron micrographs showing a range of eyg mutant phenotypes. (A) eyg¹, (B) eyg^M3-12/eyg¹, (C,D) pharate adults that failed to eclose: (C) eyg^Eq-2-d2-2/In(3LR)gv^u, most of the head structures were lost, but the antennae were present; (D) eyg^M3-12, a headless null mutant at higher magnification. (E-H) Eye-antennal disc. (E) DIC image and (F) Acridine Orange staining of the same disc from a mid-third instar eyg¹ larva. (G) BrdU labeling of an eyg¹ disc and (H) a wild-type disc from a late third instar larva. (I) Expression of P35 driven by the ey-GAL4 (abbreviated to ey>P35 in text) did not rescue the eyg¹/eyg^M3-12 phenotype. The eye disc is strongly reduced with only a few ELAV-positive cells (red), as in eyg¹/eyg^M3-12 mutants, while the antenna disc (with DAC expression, green) is of normal size. (J) eyg^M3-12 clones (marked by the absence of GFP staining; green) induced at 24-48 hours AEL were not detected in the eye disc. The heterozygous cells have nuclear GFP, while the wild-type twin-spots have a very strong GFP signal. ELAV (red) stains the photoreceptors.

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