Fig. 2. eyg encodes a Pax protein. (A) The Lune cDNA encodes a Pax protein of 670 amino acids rather than the 523 amino acids previously reported (Jun et al., 1998). The corrected Lune sequence had been deposited into GenBank (AY099362). There are three potential start codons (indicated by arrows), translating to 670, 643 and 554 amino acids, respectively. It contains a truncated Paired domain (the N-terminal extent of the truncated PAI subdomain is indicated by a wavy line; RED subdomain, residues 167-220) and a Prd-type homeodomain (HD; residues 348-407). (B) Molecular map of the eyg-toe genomic region. The 5' end of the eyg transcripts are defined by 5'-RACE. Two splicing isoforms were identified by 5'-RACE that have identical 5' ends but differ in the presence or absence of a 67 intron (GenBank AY167573 and AY167574, respectively). The exon-intron structure of eyg is indicated. Exons are indicated by black boxes. The six exons are 310, 159, 321, 141, 584 and 1072 bp, respectively. The introns are 67, 1321, 680, 125 and 211 bp, respectively. Restriction sites indicated are: BamHI (B), BglII (Bg), EcoRI (E), HindIII (H) and PstI (P). The three potential translation start sites are indicated. The toe gene is located about 30 kb downstream of eyg and has the same transcriptional orientation as eyg. (C-G) eyg expression in wild type is detected by in situ hybridization in eye-antenna disc of late third instar (C) and late second or early third instar larva (D,E), and in wing disc (F) and leg disc (G) of late third instar larva. In this and all other figures, all eye-antenna discs are oriented with anterior to the right and dorsal to the top. (H) Heat shock induction of the hs-Lune transgene can partially (center) or fully (right) rescue the eyg¹/eyg^M3-12 mutant phenotype, which is completely eyeless with complete penetrance (left).

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