Fig. 3. Molecular analysis of eyg mutants. (A) eyg^37-1, eyg^22-2 and eyg^94-4 are three eyg mutants independently derived from mobilization of the P[GawB]^EM458 (see Materials and Methods). The three lines have the P[GawB] inserted at the same location, 124 bp upstream from the eyg transcription start site, but the extent of the deletion of the flanking genomic region varies. (B) eyg and toe are both expressed in the eye and antenna discs, as detected by in situ hybridization. In the three eyg mutants, eyg RNA is progressively reduced in both the eye and the antenna discs but toe RNA is not significantly affected. The three deletion mutants also show progressively stronger eye reduction. (C) The eyg^M3-12 mutant has a 16861 bp deletion beginning at 23 bp upstream of the eyg transcription start site and extending about 13 kb downstream of eyg. The P[lacW] insert is indicated (eyg^M3-12) with the arrow showing the transcriptional direction of mini-white and lacZ. The black triangle indicates the presence of an opus retrotransposon.

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