Fig. 6. eyg and ey are transcriptionally independent. (A-D) eyg and ey expression in stage 17 embryos. In wild-type embryos, eyg (A) and ey (C) were expressed in the eye-antennal disc primordium (arrow). (B) In ey² mutant embryos, eyg expression in eye-antennal disc primordium appeared normal. (D) In embryos homozygous for the C1 deficiency (null for eyg), ey expression in the eye-antennal disc primordium appeared normal, although the head was deformed. (E) eyg expression in an ey² mutant eye-antennal disc. The ventral expression was reduced, while the dorsal expression was not significantly affected. (F,G,J,K) Ectopic dpp>eyg induced eyg expression in the dpp domains in eye (F, black arrowheads), antenna (F, arrow) and wing (J, black arrowheads) discs. The induced eyg expression was patchy along the eye disc lateral margins and did not occur in the posterior margin (F). The induced expression was much stronger than the endogenous eyg expression in the anterior notum in wing discs (J, white arrowhead) and in the antenna discs (F, white arrowhead), which is barely detectable with this staining condition. dpp>eyg did not induce ey expression in the eye-antennal (G) and wing (K) discs. (H,I,L) dpp>ey induced ey expression in the eye-antennal disc (I, arrowheads) and in wing disc (L, green), but did not induce eyg expression in the eye-antennal disc (H) and wing disc (L, red). Note the outgrowth of the antennal disc (H,I) that corresponds to the ectopic eye induced by dpp>ey. The expressions were detected by RNA in situ hybridization.

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