Fig. 5. Transcriptional profiling of tagA mutant cells. Wild-type (AX4) and mutant (tagA^-) cells were developed for 24 hours. RNA samples were collected at 2-hour intervals and analyzed with a microarray of about 6,000 genes. The data from a selected set of 2,021 developmentally regulated genes were plotted to indicate the level of gene expression where the color scale represents the standardized log₂ of the ratio between the test sample and the standard relative to the mean for each gene. Blue indicates lower than average level of gene expression for that gene and yellow indicates higher than average level of expression (Van Driessche et al., 2002). Each column represents a time point and each row represents a gene. (A) RNA from wild-type (Ax4) cells where the data are normalized with the gene means from the wild-type data (self-normalized). (B) RNA from tagA^- cells where the data from every gene are self-normalized. (C) RNA from tagA^- cells where the data from every gene are normalized to the corresponding gene mean in the wild-type (AX4) dataset. (D) The similarity (Pearson correlation) between all the genes at each time point in the AX4 dataset (y axis) and all the genes at each time point in the tagA^- dataset (x axis) was calculated. For each time of tagA^- development the most similar wild-type time point is plotted (solid line) in comparison with a theoretical plot between two identical time courses (broken line). The AX4 data set was published previously (Van Driessche et al., 2002) and was reanalyzed in the context of this experiment.

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