Fig. 8. Dorsal ventral patterning and optic fissure closure are normal in ThyCreShh^n/c embryos. (A,B,D,E) Sagittal sections through the eyes of E13 wild-type (A,B) and ThyCreShh^n/c (D,E) embryos hybridized with Vax2 (A,D) and Pax6 (B,E) riboprobes. Note the absence of coloboma (D,E), and the normal expression of Vax2 (D) in the ventral retina, and Pax6 (E) in the retina, lens, and corneal epithelium of ThyCreShh^n/c embryos in comparison with wild-type littermates. (C,F) Collagen type IV immunostaining reveals the presence of normal vascular tunics in the posterior chamber of E17 ThyCreShh^n/c eyes (compare hyaloid vessels in C and F). ac, anterior chamber; l, lens; hv, hyaloid vessels; cb, ciliary body.

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