Fig. 6. Dorsoventral patterning following Hoxa3 misexpression. (A-T) Immunostaining on transverse cryostat sections of embryos electroporated with mouse Hoxa3/tauGFP at stage 10. (A-D,E-H) Sections of two different embryos grown up to stage 27 prior to fixing. (I-P) Sections through another embryo that was fixed at stage 18. (Q-T) Sections through a second embryo fixed at stage 18. D,F,H,J-K,M-N are higher magnifications of C,E,G,I,L, respectively, showing only the electroporated side. Antibodies are as indicated. (A,E-F,J,M,Q,S) GFP natural fluorescence. K,N,R,T are the same sections as J,M,Q,S, respectively, but without the GFP signal. White asterisks (A,B,C,G,I,L,O) indicate the electroporated side. White arrows (D) indicate Islet2/Lim3-positive motoneurones. Asterisks (O,T) represent regions of repression of Pax6 and of induction of Olig2 expression, respectively. (P) r8, (S,T) r1; all other axial levels shown are r3/4. Scale bar: 120 µm for A,C,Q,T; 60 µm for D,E,G,I,L,O-P; 30 µm for F,H,J-K,M-N.

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