Fig. 4. Gnu localization in ovaries and embryos. Confocal sections of ovaries from gnu females rescued by the homozygous genomic gnu-GFP construct (stock GG4c). (A) Gnu-GFP fluorescence (green; DNA stained red) was first observed in oocytes of stage 11 egg chambers. (B) In subsequent stages, the fusion protein accumulated in the oocyte but was not observed in nurse cells. (C) Gnu-GFP fluorescence was not found to be specifically associated with the polar bodies (D). (E-F) Syncytial embryo in interphase of cycle 10. The Gnu-GFP fluorescence was cytoplasmic (E) and not excluded from nuclei (F). (G-I) Confocal sections of a giant nucleus from flies homozyogus for png³³¹⁸ and also containing a single heterozygous copy of the genomic gnu-GFP construct. (G) DNA, (H) Gnu-GFP fluorescence, (I) merged image with DNA in red and Gnu-GFP fluorescence in green. Scale bars: 100 µm (A,B), 10 µm (C-F), 20 µm (G-I).

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