Fig. 5. Gnu mis-expression in ovaries results in sterility. Confocal sections of ovaries and egg chambers from females containing (A,D-F) the maternal 4 tubulin>GAL4:VP16 and UASp gnu-GFP, (B) maternal 4 tubulin>GAL4:VP16 with UASp EGFP constructs in a wild-type background, (C) maternal 4 tubulin> GAL4:VP16 with UASp gnu-GFP in a homozygous png¹⁰⁵⁸ null background. (A) Gnu-GFP mis-expressed in ovaries is nuclear and disrupts oogenesis with inappropriate nurse cell degeneration and fragmented chromatin (arrow). (B) Ectopic GFP is cytoplasmic and nuclear and does not disrupt ovary morphology. (C) Ectopic Gnu-GFP is largely nuclear but oogenesis is not disrupted and ovarian morphology is normal. DNA is red and GFP fluorescence, green. (D-F) A single stage 8 egg chamber. (D) Propidium iodide-stained DNA, (E) GFP fluorescence, (F) merged image of D and E, with DNA in red and Gnu-GFP fluorescence in green. Not all nurse cell nuclei contain Gnu-GFP (arrowhead). Scale bars: 50 µm. (G) Anti-GFP blot shows that Gnu-GFP expressed from the GAL4-UAS system is unmodified, in contrast with that expressed from the genomic gnu-GFP construct.

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