Fig. 6. Abnormal actin reorganisation in ovaries mis-expressing Gnu. (A) Propidium-stained DNA, (B) FITC-phalloidin stain for F-actin. (C) Merged image of A and B. DNA red, F-actin green. (D) Gnu-GFP in nurse cell nuclei. (E) Rhodamine-phalloidin stain for F-actin. (F) Merged image of D and E Gnu-GFP green, rhodamine-phalloidin red. Gnu-GFP mis-expression was compatible with some aspects of F-actin organisation, such as ring canals (E arrowhead, F). At the equivalent of wild-type stage 10, the actin cytoskeleton aggregated (E arrow) and did not develop the contractile meshwork (B arrowhead, C) that in the wild type ovary dumps nurse cell cytoplasm into the oocyte and retains nurse cell nuclei in oogenic stages 10B and 11. Confocal sections were taken of egg chambers from females containing the maternal 4 tubulin>GAL4:VP16 (A-C) and UASp gnu-GFP (D-F) constructs in wild-type background.

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