Fig. 4. Stbm co-immunoprecipitates Dsh and Pk. Immunoprecipitations from COS7 cells transfected with the plasmids indicated. Proteins in the lysate prior to immunoprecipitation are shown on the left (input), and either five times (B-D) or 10 times (A,E,F) equivalent immunoprecipitated material is on the right. (A) Dsh-GFP co-immunoprecipitated with FLAG-Stbm. (B) Myc-Pk co-immunoprecipitated with FLAG-Stbm. (C,D) Lysate containing FLAG-Stbm, Dsh-GFP and Myc-Pk immunoprecipitated with anti-FLAG co-immunoprecipitates both Dsh-GFP (C) and Myc-Pk (D). (E) Weak co-immunoprecipitation of Dsh-GFP by Myc-Pk. (F) Myc-Pk co-immunoprecipates a fusion of CD2 extracellular and transmembrane regions to Stbm C-terminal intracellular domain lacking the putative PBM. Arrows indicate specific bands of expected molecular weights detected by western blotting for co-immunoprecipitated proteins.

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