Fig. 5. Stbm recruits Dsh and Pk to membranes. Confocal images of COS7 cells transfected with the plasmids indicated (A-Q), or third instar wing disc expressing Stbm-YFP (R). Distribution of proteins expressed singly is shown in white. Stbm (detected with anti-Stbm), FLAG-Stbm (detected with anti-FLAG) and Stbm-YFP (detected by YFP fluorescence) is always shown in green in multiply labelled panels. Dsh (detected with anti-Dsh) is always shown in red in multiply labelled panels. Pk (detected with anti-Pk) and Myc-Pk (detected with anti-Myc) are always shown in blue in multiply labelled panels. Stbm-PBM (K-M) lacks the last three amino acids of the ORF. CD2-Stbm-Cterm-PBM (N-Q) consists of the extracellular and transmembrane regions of rat CD2, fused to the C-terminal intracellular tail of Stbm with the last three amino acids deleted. (A-C) Stbm (white in A, green in C) can recruit Dsh (white in B, red in C) from cytoplasmic vesicles to Stbm-expressing membranes (largely the Golgi membranes but partly the outer cell membrane). (D-F) FLAG-Stbm (white in D, green in F) can recruit Pk (white in E, blue in F) to Stbm-expressing membranes. (G) FLAG-Stbm (green), Dsh (red) and Pk (blue) colocalise to Stbm expressing membranes. (H) Dsh (red) and Pk (blue) co-expressed together do not colocalise. (I,J) Recruitment of Dsh (white in I, red in J) to the outer membrane by Fz (I) is not disrupted by co-expression of Pk (blue in J). (K-Q) Stbm-PBM (white in K, green in L,M) or CD2-Stbm-Cterm-PBM (white in N, green in O,Q) can recruit Dsh (red in L,O) and Pk (white in P, blue in M,Q). (R) Stbm-YFP and Dsh colocalise apicolaterally in the wing pouch of a third instar wing disc.

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