Fig. 5. PointedP2 is necessary and sufficient to repress cardioblast development. Dorsal whole heart views of stage 15 wild-type (A), PntP2 RNAi embryos (B-D) and pnt⁰¹²³⁰⁹ embryos in which either pntP1 (E) or pntP2 (F) was expressed throughout the mesoderm labeled for Mef2 (A,B,D-F) or PntP1 (C). (A) Wild-type embryo showing the two bilateral rows of cardioblasts (arrows). (B) PntP2 RNAi-treated embryo exhibiting a significant increase in cardioblasts (arrows). (C,D) pntP2 RNAi-treated embryo double-labeled for pntP1 (C) and Mef2 (D). This embryo expresses PntP1 in essentially a wild-type pattern (C) and exhibits a significant increase in cardioblasts (arrows, D). (E) pnt⁰¹²³⁰⁹ embryo in which pntP1 has been expressed ubiquitously throughout the mesoderm exhibits a drastic loss of cardioblasts (arrows indicate cardioblasts). (F) pnt⁰¹²³⁰⁹ embryo in which PntP2 has been expressed ubiquitously throughout the mesoderm exhibits wild-type cardioblast development (arrows). Anterior is towards the left.

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