Fig. 3. Pan-mesodermal expression in progeny of the cross between twi-Gal4;24B-Gal4 driver and UAS-cDNA containing transgenic flies. (A-F) tin expression in late-stage 11 embryos. (A) Wild type. (B) UAS-pnr embryo shows ectopic expression in the ventrolateral mesoderm (arrow). (C) UAS-ush embryo shows unaltered or slightly reduced tin expression (arrows). (D) UAS-ush,UAS-pnr embryo shows a moderate reduction in tin expression (arrow). (E) UAS-pnrD4 shows an increase in tin expression in the ventrolateral mesoderm, similar to UAS-pnr embryos (B). (F) UAS-ush;UAS-pnrD4 embryo shows an increase in tin expression in the ventrolateral mesoderm, similar to UAS-pnrD4 embryos (E). (G-L) tin expression in stage 13 embryos. (G) Wild type. (H) UAS-pnr embryo shows moderate ectopic expression in the ventrolateral mesoderm (arrows). (I) UAS-ush embryo shows a moderate reduction in tin expression. (J) UAS-ush,UAS-pnr embryo shows a similar decrease in tin expression as in UAS-ush embryos (I). (K) UAS-pnrD4 embryo shows dramatic ectopic expression in the ventrolateral mesoderm. (L) UAS-ush;UAS-pnrD4 embryo shows a similar increase in ectopic tin expression as in UAS-pnrD4 embryos (K). (M-O) Hand expression in stage 13 embryos. (M) Wild type. (N) UAS-pnr embryo shows moderate ectopic expression in the ventrolateral mesoderm, as with tin (H). (O) UAS-tin;UAS-pnr embryo shows an increase in ectopic Hand expression in the ventrolateral mesoderm that is comparable with tin expression in embryos with mesodermal overexpression of UAS-pnrD4 (K).

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