Fig. 3. ci mutant clones in the eye disc do not block Eya expression, MF initiation, progression or photoreceptor differentiation. (A,B) Each set of four panels in A and B show the same eye disc containing ci mutant clones stained with (A) anti-ß-galactosidase, anti-Ci, anti-Senseless and a merge of all three channels or (B) anti-ß-galactosidase, anti-Ci, anti-Eya and a merge. The yellow arrows in A and B mark clonal areas. No obvious disruption in Eya or Senseless staining (A) is observed in ci-null mutant clones. (C) Thin plastic sections of adult eyes containing ci mutant tissue. Mutant clones are negatively marked by the lack of pigment granules. Photoreceptor differentiation is normal even in very large clones of ci mutant tissue. (D,F) Animals heterozygous for ci⁹⁴ or homozygous for ci⁹⁴, but rescued by one copy of the P[ci⁺] transgene, have normal Ci staining in the wings disc (D) and adult wings (F). (E,G) By contrast, induction of mutant clones in the anterior compartment of the wing disc leads to loss of Ci in the wing disc (arrow, E) and disruption of pattern in the anterior adult wing disc (arrow in G).

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