Fig. 4. Skeletal abnormalities and decreased bone density in Fgfr2^cko mice. (A,B) Alizarin Red stained skulls from P30 control (A) and Fgfr2^cko (B) mice, showing a dome-shaped skull in the Fgfr2^cko mouse. (C,D) Dorsal view of the Alizarin Red-stained axial skeleton from a P30 control (C) and Fgfr2^cko (D) mouse, showing a non-ossified gap in the midline of the vertebrae and the absence of the spinous process (arrows) in the Fgfr2^cko mouse. (E,F) Alizarin Red- and Alcian Blue-stained tarsal bones from a P7 control (E) and Fgfr2^cko (F) mouse. All of 13 mice examined showed tarsal bone fusion (arrows). (G,H) Alizarin Red stained tarsal bones from a P60 control (G) and Fgfr2^cko (H) mouse. The cuneiforme 3 bone is fused with naviculare and cuboideum (arrows) in the Fgfr2^cko mice. Note that in the adult, the cuneiforme 2 and 3 bones are also fused in the Fgfr2^cko mice (arrows). (I,J) Radiological analysis of bones from Fgfr2^cko mice and normal littermates showing decreased bone length and increased radiolucency in Fgfr2^cko mice. (I) Femurs, P22; (J) femurs, P43; (K) quantitative analysis of bone mineral density by DEXA of femur (black) and lumbar vertebra (red) of mice age 1-58 weeks. Solid bars, control mice; open bars, Fgfr2^cko mice. ^*P<0.05.

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