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Fig. 3. Six1 is required for the expression of Pax2 and Six2 but not Eya1 in the metanephric mesenchyme (mm) at E10.5. (A-D) Pax2 is normally expressed in the intermediate mesoderm (A,B), the metanephric mesenchyme before and after induction and in the ureteric epithelium (ub). In Six1-/- embryos, however, its expression in the metanephric mesenchyme at E10.5 is undetectable (arrows in D). (E,F) Eya1 is expressed in the metanephric mesenchyme before and after induction and its expression is not affected in Six1-/- mesenchyme at E10.5. (G,H) Six2 is also expressed in the metanephric mesenchyme before and after induction and its expression is undetectable in Six1-/- mesenchyme at E10.5 (arrows).





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