Fig. 3. spen is required for maximal Wg-dependent repression of proneural genes in the eye. (A-D) Confocal images of a P[sev-wg] pupal eye (3-6 hours after pupal formation–APF) containing spen³ clones generated by ey-FLP. Clones of spen³ were marked by the absence of GFP (A; clonal boundaries shown by the white lines in B-D) and were stained for Ac (B) and Wg (C); the merged image is shown (D). P[sev-wg] eyes have high levels of Wg behind the morphogenetic furrow (C) and low levels of Ac (B) outside the spen³ clones. Ac is derepressed in much of the clone (B; note that cells in and ahead of the MF are not competent to express Ac) but significant Ac repression still occurs inside the clone (arrows in B). Transgenic Wg levels are unaffected in spen³ clones (C). Similar Ac derepression and P[sev-wg] expression are also seen in spen^14C2 clones (data not shown). Occasional non-autonomous derepression of Ac is observed adjacent to spen clones (B, arrowhead). (E,F) Confocal images of a pupal eye (3-6 hours APF) containing spen^14C2 clones generated by ey-FLP. Clones of spen^14C2 were marked by the absence of GFP (E) and were stained for Ac (F). Ac expression is elevated in spen^14C2 clones (arrows in F; note that the laser intensity is lower than in B).

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