Fig. 6. spen is required for Wg signaling in the leg imaginal disc. (A-I) Confocal images of third instar leg imaginal discs of wild type (A-C) and animals containing transgenes P[ptc-Gal4], P[dpp-lacZ] and P[UAS-spen^DN]^II (D-I) reared at 21°C. Samples were stained for Wg (A,D,G) and lacZ (B,E,H); merged images are shown (C,F,I). All panels are shown with the same magnification. Ventral (v) expression of Wg (A) restricts lacZ expression to the dorsal (d) half (B) in the wild type. Expression from P[UAS-spen^DN] along a stripe overlapping both the wg and dpp domains either has no effect on the restriction of lacZ expression (D-F) or leads to derepression of lacZ in the ventral region (G-I; note the overlap of Wg and lacZ in I; 9% are similar to I, 45% have significant but incomplete derepression of lacZ, and 46% are similar to F, n=35). This derepression is consistent with a block in Wg signaling.

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