Fig. 2. unc-71 expression pattern. (A-C,E) Expression patterns of Punc-71GFP. GFP is seen in the excretory cell (arrow in A), the excretory gland (arrow in B), in some neurons in the head (arrowhead in B), one of which is AVG interneuron (arrow in C), and in hypodermis (arrow in E) flanking the vulva in adult animals. (D) Punc-71GFP is not expressed in the ventral cord motoneurons; the GFP present is from the processes of the interneurons in the head (arrow points to the ventral cord). (F,G) UNC-71::GFP may be localized to the cell membrane. Punc-115UNC-71::GFP expression is seen in the nerve ring (arrow in F) and along the nerve cords(arrow in G). (H,I) P_e15^*₂UNC-71::GFP expression in hypodermis is seen near the nerve cords (arrow in H,I). Arrowhead in H indicates the vulva hypodermis. ^*Likely GFP aggregates. Scale bars: 20 µm in A,F-I; 50 µm in B-E.

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