Fig. 9. Ectopic expression of Dorsocross represses wingless. (A) wg expression in stage 11 control embryo (UAS-Doc2/CyO). (B) Embryo of similar stage expressing Doc2 ectopically in the whole ectoderm (e22c-GAL4/UAS-Doc2). Ventral wg expression is missing (see arrowhead) and dorsal wg expression is almost continuous, although with reduced levels. (C,D) Dorsocross (red) and wg (green) expression in imaginal wing discs from 3rd instar larvae detected by flourescent double-staining using anti-Doc2+3 and anti-Wg antibodies. Dorsal is upwards and anterior towards the left. (C) Wild-type wing disc. (D) Wing discs ectopically expressing Doc2 in the dpp expression domain (UAS-Doc2/+;dpp.blk1-GAL4/+). Note the interruption of wg expression at the intersection of ectopic Doc2 and Wg stripes (arrowheads). (E,F) Leg disc of 3rd instar stained as in C and D. (E) Wild-type leg disc with wg expression in anterior/ventral territories. Endogenous Doc protein in dorsal proximal areas of the disc is detected at low levels (red arrowheads). (F) Leg disc from UAS-Doc2/+;dpp.blk1-GAL4/+ larvae. wg expression is repressed by ectopic Doc2 in the central area that normally produces distal parts of the leg (arrowheads). (G) Wild-type wing. (H) Wing derived from a disc with a genotype as in D, which lacks distal structures (arrowheads). Wing veins appear broadened as well. (I) Adult male fly showing a phenotype of intermediate strength caused by ectopic dpp.blk1-driven Doc2. Arrowheads indicate aristaless antennae and shortened legs.

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