Fig. 3. wg downstream components are required for SSC maintenance. Germaria are labeled for lacZ (red), Hts (green), nuclei (blue). All the micrographs represent one confocal cross section of the germarium. Marked wild-type (A,B) or mutant (C-E) clones are outlined and are identified by loss of lacZ expression. (A) A germarium showing a one-week-old wild-type SSC clone and its follicle cell progeny (outlined). The putative marked SSC is indicated by an arrowhead. (B) A germarium showing a three-week-old wild-type SSC clone in which all follicle cells (outlined) are marked, indicating that all the SSCs are marked. One of the putative marked SSCs is indicated by an arrowhead. (C) A germarium showing a one-week-old mutant dsh^VA135 SSC clone (outlined). The putative marked dsh mutant SSC is indicated by an arrowhead. (D) A germarium showing loss of a marked mutant dsh^VA135 SSC clone three weeks after clone induction indicated by absence of marked follicle cells in the germarium but their presence in a late egg chamber (outlined in the inset). (E) A germarium with a marked mutant sgg clone three weeks after clone induction. (F) A germarium with all SSCs mutant for sgg three weeks after clone induction, indicated by loss of lacZ expression in all the follicle cells. All the germaria are shown at the same scale. Scale bar in A: 10 µm. SSCs, somatic stem cells.

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