Fig. 4. wg signaling is not required for the proliferation of follicle cells on egg chambers. All egg chambers are labeled for lacZ (red), Hts (green) and nuclei (blue). The micrographs in A and B represent one confocal cross section of an egg chamber, whereas the micrograph in C is one confocal section along the surface of an egg chamber. Marked mutant follicle cell clones are identified by loss of lacZ expression (outlined in panels A-D). (A) A stage-8 egg chamber carrying marked mutant arm² follicle cell clones. The arm² mutant follicle cells have normal accumulation of Hts in lateral membranes. (B) A stage-6 egg chamber carrying mutant arm⁴ follicle cell clones in which Hts accumulates abnormally on apical membranes. In this egg chamber the oocyte is mislocalized to the anterior end because of a defect in DE-cadherin-mediated cell adhesion. However, the mutant follicle cell clones appear to have a normal size. (C) A stage-10 egg chamber showing a mutant dsh^VA135 follicle cell clone. The mutant clone appears to have a normal size and normal expression of Hts on lateral membranes. (D) A stage-9 egg chamber carrying twin follicle cell clones. The mutant dsh follicle cell clone, which is identified by loss of lacZ expression and highlighted by broken lines, appears to proliferate normally in comparison with the corresponding wild-type twin clone. The wild-type clone (highlighted by a solid line) carries two copies of the lacZ gene and thus shows stronger lacZ expression than the rest of lacZ-positive follicle cells (carrying one copy of the lacZ gene). The mutant clone appears to have a normal size and normal expression of Hts on lateral membranes. Scale bars: 10 µm.

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