Fig. 1. Functional complementation of the yeast smc2-6 temperature sensitive mutant with the AtCAP-E1 cDNA. (A) Diagram of the position on the plates of the cell types in B and C. (B) Rescue of mutant cells transformed with AtCAP-E1 cDNA in the presence of galactose at 37°C. (C) smc2-6 mutant cells transformed with AtCAP-E1 cDNA failed to grow at 37°C in the absence of galactose. (D,E) DAPI-stained mutant and transformed cells, respectively, exhibited the cut phenotype in the absence of galactose at 37°C. (F,G) Wild-type and transformed cells, respectively, showed normal chromosome segregation in the presence of galactose at 37°C.

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