Fig. 2. ASY1 immunolocalization during meiosis in wild type and swi1-2 mutant. In each panel, the upper picture shows a DAPI-stained nucleus (blue) and the lower one shows the ASY1 immunolocalization (green) on the same nucleus. (A-C) Wild-type meiotic nuclei. (A) Pre-leptotene nucleus, ASY1 is associated with chromatin. (B) Leptotene, ASY1 localization stains the axial elements. (C) Pachytene, ASY1 localizes along synapsed chromosomes. (D-I) swi1-2 meiotic cells. (D,E) ASY1 localizes on chromatin as it does in wild type. (F,G) ASY1 is present on the condensing ten univalents. (H) The sister chromatid cohesion is released, resulting in 20 free chromatids on which ASY1 is still present. (I) A polyad composed of nine micronuclei on which ASY1 localizes. Scale bars: 10 µm.

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