Fig. 1. Synergism between mutations in lin-35 and ubc-18. DIC (A) and corresponding GFP fluorescence (B) images. The large adult with GFP fluorescence is of genotype lin-35; ubc-18; kuEx119. The white arrows indicate the position of an arrested lin-35; ubc-18 L1 larva that failed to inherit the array; the white arrowheads indicate a sterile lin-35; ubc-18 young adult. Inset in A shows an arrested lin-35; ubc-18; KuEx119 larval-stage animal treated with lin-35(RNAi) (same animal is shown in the inset in B). (C,D) L1 animals of genotypes lin-35; ubc-18 and wild type, respectively. White and black arrowheads indicate anterior and posterior pharyngeal boundaries, respectively. Note the failure of the mutant pharynx to extend to the anterior end of the animal. (Inset in D) myo-2::GFP fluorescence in the wild-type background, highlighting the bi-lobed shape of the pharynx. Scale bars: in A, 100 µm for A,B; in C, 10 µm for C; in D, 10 µm for D.

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