Fig. 3. pha-4::GFP expression in wild-type and mutant animals. DIC (A,C,E,G) and corresponding pha-4::GFP fluorescence (B,D,F,H) images. (A-D) 1.5-fold-stage wild-type (A,B) and ubc-18; lin-35(RNAi) mutant (C,D) embryos. Anterior is towards the left and ventral is downwards. Arrows indicate the dorsoventral pharyngeal boundaries. Note gross differences in pharyngeal shape and borders in double mutants versus wild-type embryos. In addition, note the overall similarity in the numbers of GFP-expressing cells in wild-type and double-mutant embryos. (The mutant and wild-type embryos contained 102 and 101 GFP+ nuclei, respectively.) The white bracket (D) demarcates the presence of several GFP-expressing arcade cells that failed to integrate with the rest of the pharynx. Intestinal cell nuclei, which are large, round and reside to the posterior, can readily be distinguished from pharyngeal nuclei at this stage. (E-H) pha-4::GFP expression in wild-type (E,F) and ubc-18; lin-35(RNAi) mutant L1 larvae (G,H). White and black arrowheads indicate anterior and posterior pharyngeal boundaries, respectively. As above, note the overall similarity in the number of GFP-expressing cells in contrast to the gross differences in pharyngeal shape between wild-type and double-mutant animals. The bracket (H) indicates the non-integrated arcade cells. Scale bars: in A, 10 µm for A-D; in E, 10 µm for E-H.

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