Fig. 4. Expression of differentiation markers in mutant animals. DIC (A,C,E) and corresponding GFP fluorescence (B,D,F) images of ubc-18; lin-35(RNAi) mutant animals. White and black arrowheads indicate anterior and posterior pharyngeal boundaries, respectively. (A,B) Expression of the differentiated muscle cell marker, myo-2::GFP. (C,D) Expression of the adherens junction component and marker for epithelial cell differentiation, ajm-1::GFP. Bracket indicates the region of ajm-1 expression in the non-extended pharynx. (E,F) Neuronal marker (unc-119::GFP) expression in a pharynx that underwent partial extension. White arrows (F) indicate the positions of several GFP+ nuclei that lie within the pharyngeal borders. Scale bar: 10 µm.

Return to article