Fig. 1. Genomic structure of ceh-2 and sequence comparisons. (A) Alignment of homeodomains and hexapeptides of ems class genes from various species. Dots designate identical amino acids, vertical lines indicate intron positions and dashes denote artificial gaps that are introduced for alignment purposes. Abbreviations and sources of sequence data: Bf, Branchiostoma floridiae (amphioxus) (AF261146) (Williams and Holland, 2000); Dm, Drosophila melanogaster (X51653, X66270) (Dalton et al., 1989; Walldorf and Gehring, 1992); Dr, zebrafish (D32214, D32215) (Morita et al., 1995); Hs, human (X68879, X68880) (Simeone et al., 1992); Hys, Hydractinia symbiolongicarpus (cnidarian) (Mokady et al., 1998); Nv, Notophthalmus viridescens (newt) (Beauchemin et al., 1998); Mm, mouse (X68881, X68882) (Simeone et al., 1992); Xl, Xenopus laevis (Pannese et al., 1998). (B) Genomic structure of the ceh-2 gene and flanking ORFs on cosmid C17A12. The extent of the ch4 deletion is indicated underneath the gene. Rescue subclones (pTRB203, pTRB204) and reporter constructs (pTRB201, pTRB202), as well as the homeodomain (HD), hexapeptide (HP) and the position of the peptide antigen are shown. Grey boxes indicate acidic regions.

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