Fig. 5. Cell proliferation and survival are not altered significantly in Fgf3^-/-;Fgf10^-/- embryos. Whole mount embryos were analysed for mitotic or apoptotic cells using an antibody to phosphohistone H3 (A,C) or TUNEL (E,G), respectively and sectioned in the transverse plane. A section taken through the otic region (the plane is indicated by a line through each embryo) is shown in the panel to the right of each whole embryo. Rostral is to the left. Phosphistone H3 immunoreactivity in transverse sections from an 8 somite control (B) and double mutant embryo (D). Mitotic cells (brown) can be identified in all three tissues that are involved in otic induction; neurectoderm (ne), mesoderm (m) and surface ectoderm (se). TUNEL staining in transverse sections from 7 somite control (F) and double mutant embryos (H). Apoptotic cells (brown) can be identified in all three tissues that are involved in otic induction; neurectoderm, mesoderm and surface ectoderm.

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