Fig. 7. Analysis of the area and position of the otic vesicle in embryos with three mutant Fgf alleles reveals quantitative differences between Fgf3^+/-;Fgf10^-/- and Fgf3^-/-;Fgf10^+/- embryos. (A) Quantitative comparisons between the ratio of the area of the central otic vesicle section to the area of the central eye section in Fgf3^+/-;Fgf10^+/-, Fgf3^+/-;Fgf10^-/- and Fgf3^-/-;Fgf10^+/- embryos (n=6 ears and eyes per genotype). The average ear area/eye area (in %) is shown on the y-axis. The genotypes analysed are shown on the x-axis. The otic to optic area ratios of the Fgf3^+/-;Fgf10^-/- and Fgf3^-/-;Fgf10^+/- samples were significantly lower than that of the Fgf3^+/-;Fgf10^+/- embryos (P=0.005 and P=0.001, respectively). (B) Quantitative analysis of the dorsal-ventral position of the otic vesicle. The vertical distance from the dorsal surface of the neural tube to the top of the otic vesicle was measured and compared to the dorsoventral length of the neural tube in Fgf3^+/-;Fgf10^+/-, Fgf3^+/-;Fgf10^-/-, and Fgf3^-/-;Fgf10^+/- embryos (n=6 ears per genotype). The dorsoventral (DV) distance to the otic vesicle/dorsoventral length of the neural tube is shown on the y-axis. The genotypes analysed are shown on the x-axis. The position of the otic vesicle in Fgf3^-/-;Fgf10^+/- embryos was significantly ventralised relative to that in Fgf3^+/-;Fgf10^+/- embryos (P=0.001). The asterisk indicates a significant paired t test result between the genotype shown and Fgf3^+/-;Fgf10^+/- embryos. The bar above each data cell indicates one s.d. 3, Fgf3; 10, Fgf10.

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