Fig. 2. Regulation of Ptx1 expression by Fgf. Early E9.5 branchial arches were dissected out and incubated for 24 hours on isopore filters, and Ptx1 expression was determined by in situ hybridization (A-F,I,J), or apoptosis was determined by TUNEL (G,H). (A) Wild-type first arch mesenchyme incubated without epithelium. (B) Wild-type first (I) and second (II) arches incubated with epithelium. (C) Wild-type first and second arches incubated without epithelium in the presence of a bead soaked in PBS. (D) Wild-type first and second arches incubated without epithelium in the presence of a bead soaked in 1 mg/ml Fgf8. (E) Wild-type first and second arches incubated with epithelium in the presence of a bead soaked in DMSO. (F) Wild-type first and second arches incubated with epithelium in the presence of a bead soaked in 13.5 mM SU5402. There is only residual Ptx1 expression in the first arch. (G) TUNEL assay on wild-type first and second arches incubated with epithelium in the presence of a bead soaked in DMSO. (H) TUNEL assay on wild-type first and second arches incubated with epithelium in the presence of a bead soaked in 13.5 mM SU5402. (I) Hoxa2^-/- first and second arches incubated with epithelium. (J) Hoxa2^-/- first and second arches incubated with epithelium in the presence of a bead soaked in 13.5 mM SU5402.

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