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Fig. 4. Lhx6, Fgf8, Hoxa2 and Bmp4 expression in the branchial area. (A,B) Expression of Lhx6 in E10.5 wild-type (A) and Hoxa2-/- (B) embryos. The arrow indicates the extra expression domain in the second arch mesenchyme beneath the rostral epithelium. The inset in B shows a section through the Lhx6-positive domain of the Hoxa2 mutant second arch, to demonstrate that expression is in the mesenchyme. (C,D) Expression of Fgf8 in E 10.5 wild-type (C) and Hoxa2-/- (D) embryos. The arrow indicates expression in the epithelium of the first cleft/pouch. (E) Expression of Hoxa2 in E10.5 embryos. The arrow indicates the strong expression in the mesenchyme beneath the cleft/pouch epithelium. (F) Expression of Bmp4 in E9.5 wild-type embryos. The arrow indicates the expression in the cleft/pouch.





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