Fig. 8. Neuronal and radial glial cells at 48 hpf. (A) Transverse section at 48 hpf labelled randomly with -tub Gal4 VP16 UAS GFP. In this specimen we see a single neuron with growth cone (arrowed) and a single cell with a radial glial-like morphology. Scale bar: 50 µm. (B) Higher magnification view of cells seen in A. Arrowheads point to radial glial processes extending to both the ventricular and pial surfaces. (C) Transverse section of hindbrain at the level of rhombomere 5. Confocal projection of neurons labelled at random with -tub Gal4 VP16 UAS GFP. Scale bar: 50 µm. (D) Confocal section through the hindbrain at 48 hpf double-stained with anti-phosphohistone H-3 (green) and anti-zrf-1 (red). Scale bar: 50 µm. (E) Higher magnification of cells expressing both phosphohistone H-3 and zrf-1. (F) Confocal section through the hindbrain at 48 hpf stained with anti-GFAP (green). Scale bar: 50 µm. (G) High magnification of a single cell expressing both phosphohistone H-3 (yellow) and anti-GFAP (green).

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