Fig. 6. Cell-type-specific requirements for obe and leo. (C,F,I,L) Diagrammatic representation of the outcomes of the mosaic analyses. Melanophores are represented in blue, xanthophores in yellow. Wild-type cells are shaded darker than mutant ones. (A-C) Transplantation of golden bpeGFP cells into nac;obe double mutant hosts generates a wild-type pattern. (A) A clone of wild-type melanophores organising the surrounding xanthophores into stripes of wild-type appearance. (B) A variety of cell types displaying GFP expression in the vicinity of the clone, but the majority of xanthophores is GFP negative and thus mutant for obe (arrows). (D-F) Upon transplantation of obe; bpeGFP cells into nac mutants, an obe like pattern is formed. (D) A melanophore clone with loosely clustered melanophores and intermingled xanthophores. (E) Only a few xanthophores are GFP positive (arrowhead), whereas the majority only displays autofluorescence (arrow) and is thus wild type for obe. (G-I) Transplantation of bpeGFP cells that are wild type for leo into nac;leo hosts generates a leo like pattern. (G) A clone of wild-type melanophores displaying key features of the leo pattern such as undulating interrupted stripes and spots. (H) Many different GFP positive cells are discernible around the clone, but most of the xanthophores are GFP negative (arrows) and thus mutant for leo. (J-L) Transplantation of leo; bpeGFP cells into nac single mutant hosts results in a leo like pattern. (J) A clone of leo mutant melanophores forming spots and undulating stripes. (K) Both GFP positive leo mutant xanthophores (arrowheads) as well as GFP-negative, wild type xanthophores (arrows) participate in formation of leo like pattern elements.

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