Fig. 3. tai exhibits dominant genetic interactions with Pvf1. (A) PZ8685 staining in a tai^61G1/CyO egg chamber at stage 10. The border cells (arrow) have completed their migration and reside at the anterior border of the oocyte (arrowheads). The dashed line indicates the distance 50% along the length of the migration route. The dotted line indicates the distance 90% along the length of the migration route. The border cells in this egg chamber have migrated more than 90% of the normal distance. (B) PZ6356 staining, which labels the border cells, the oocyte nucleus and some nurse cell nuclei, in a Pvf1^EP1624/+; tai^61G1/+ double heterozygous egg chamber at stage 10. Border cell migration (arrow) is delayed. In this egg chamber, the border cells have migrated 60% of the normal distance. (C,D) Quantitation of border cell migration shown as the proportion of egg chambers in which the border cells migrated 0-25% (yellow), 26-50% (blue), 51-75% (red) and 76-100% (black) of the normal distance. Border cell migration was assayed in the indicated genotypes at stage 10. The number of egg chambers examined is indicated (n). (C) Interaction of tai with Pvf1^EP1624 (EP1624). (D) Interaction of tai with slbo-GAL4/UAS-Pvf1.

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