Fig. 5. DMKP3 affects different cellular decisions from PTP-ER. (A) Section through Dmkp3 mutant eyes reveals misdifferentiations affecting the outer R3 and R4 cells, as well as a central cell that is normally not incorporated into ommatidia. Yellow dots mark mutant ommatidia and the different types and corresponding percentages are shown on the right. (609 ommatidia were analyzed, 4% of which could not be unambiguously assigned.) 1, Wild-type; 2-6,8,9, symmetrical ommatidia; 2,7-9, with an extra R7; 6, containing an extra outer photoreceptor; 2,4,8, R3/R3-type; 5,9, R4/R4 type; 3,7, devoid of an R3/R4-type photoreceptor; 4 and 5 are sometimes hard to distinguish and therefore percentages are combined. Cartoons to the right symbolize the different mutant classes with R3 rhabdomeres in red, R4 rhabdomeres in green and rhabdomeres of unclear identity in yellow. (B) The eye phenotype is almost completely suppressed by rl^10A. The only mutant ommatidium out of 700 is marked. (D) In Dmkp3^- eye imaginal discs two (arrows) or no (arrowhead) cells per cluster (green) may differentiate into R4 cells (red, single channel in C). (E) PTP-ER^- eyes are rough because of the recruitment of extra R7 cells (red dots), and PTP-ER^- Dmkp3^- pharate adults display ommatidia characteristic of either single mutant (yellow and red dots in F). (G) The double mutant is viable when rl/ERK dose is reduced and shows a wing phenotype characteristic of enhanced ERK activity.

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