Fig. 3. Fate mapping the mid-primitive streak. DiI/CRSE (red) and CFSE (green) injections at mid-primitive streak level (red) and an adjacent rostral level (green) at stages 3a/b (A-G), 3c (H-L), 3d (M-P) and 4 (Q-W), respectively. (A,H,M,Q) t=0 hours of reincubation. (B,C,I,N,R) t=6 hours of reincubation. (D,J,O,S,U) t=9 hours of reincubation. (E-G,K,L,P,T,V,W) t=18-24 hours of reincubation. (Note that two stage 4 embryos are shown at 9 hours (S,U) and subsequently at 18-24 hours (T and V,W, respectively). Lines in B,E,K,V indicate levels of sections shown in C,F,G,L,W. (F,K,L,W) Immunocytochemistry with anti-rhodamine (brown) and anti-fluorescein (purple) antibodies. In C, arrow indicates the midline dorsal surface cells of the primitive streak (see Lawson and Schoenwolf, 2001a; Lawson and Schoenwolf, 2001b). cip, cranial intestinal portal; ep, epiblast; np, neural plate; nt, neural tube.

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