Fig. 6. Labeling the hypoblast and the rostral primitive streak. In situ hybridization with a Crescent RNA probe (purple), and immunocytochemistry with anti-rhodamine antibody (brown), 4 hours after injection of DiI/CRSE site c_r at stage 2. (A) Crescent-labeled hypoblast in rostral half of area pellucida. (B) Crescent-labeled hypoblast as in a (purple) and rostral endoderm (brown). Line in B indicates the level of section shown in C. ep, epiblast. Note the intermixing of cells in the lower layer at the interface between the hypoblast (purple; arrowheads) and definitive endoderm (brown; arrows).

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