Fig. 3. Effects of Wnt overexpression on fibre-type differentiation in vitro. (A-P) Fluorescent images of stage 21/22 wing micromass cultures showing terminally differentiated myogenic cells that have been visualized with antibody A4.1025, which recognizes both slow and fast MyHCs (A-H, green), and antibody A4.840, which specifically recognizes slow MyHCs (I-P, red). The micromass cultures have been infected with control RCAS(BP) virus (A,I) or retroviruses expressing Wnt3a (B,J), Wnt4 (C,K), Wnt5a (D,L), Wnt6 (E,M), Wnt7a (F,N) Wnt11 (G,O) or Wnt14 (H,P). Scale bars, 100 µm.

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