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Fig. 6. Effects of loss-of-function of Wnt signalling in vivo. Fluorescent images
of transverse cryosections of day 8 chick wings that were infected with
retroviruses expressing Sfrp2 (B,D) and
Wnt11 (F,H) at stage 18-20, and
the contralateral control wings (A,C,E,G), which have been visualized with
antibody A4.1025, which recognizes slow and fast MyHCs (A,B,E,F, green), and
antibody A4.840, which recognizes slow MyHC (C,D,G,H, red). (A-D) Sections
through the whole wing. (E-H) High power pictures of the EMU and EDC muscles.
The arrows in (A,B,G,H) compare equivalent muscles, showing the changes in
muscle development. In (B), the muscles are absent (EMU, SUP) or are decreased
in size (EMR, FCU). In H, the EMU muscle has more slow fibres. EDC, extensor
digitorum communis; EIL, extensor indicis longus; EMR, extensor metacarpi
radialis; EMU, extensor metacarpi ulnaris; FDP, flexor digitorum profundus;
SUP, supinator. Scale bars: 200 µm in AD; 100 µm in E-L.