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Fig. 5. Signaling by P1 descendants. (A-D) Nomarski photomicrographs showing the division of ABpr in (A) wild-type and (B) mom-2(or42) embryos, and the division of ABpl in (C) wild-type and (D) mom-2(or42) embryos; the asterisk indicates a division into the focal plane. (E) GFP expression of an endoderm-specific transgene after combining isolated EMS and C cells; DAPI staining of the same cells is shown in F. At the time of fixation the EMS daughter distal to C had divided twice, whereas the proximal EMS daughter divided only once. (G) POP-1 expression after the division of combined AB4 and MS cells; DAPI staining is shown in H. (I) POP-1 expression after the division of the AB4 cells in a devitellinized embryo. Prior to cell division, a graft E cell was placed on one of the AB4 cells. Note that AB4 daughter proximal to E has low POP-1. J shows superimposed DAPI and PKL-2 images with arrows indicating midbodies.





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