DEV00584 Supplemental Data

Files in this Data Supplement:

• Figure S1 - Genotyping by PCR shows the absence of wild-type Pin1 allele in homozygous Pin1-deficient mice. No Pin1 protein is detected by western blot in tissue extract of Pin1^–/– mice.

• Figure S2 - Immunostaining for Pin1 (green) in 7.5 (D) and late 8.5 dpc (A) embryo sections show that Pin1 is expressed in PGCs and somatic cells at those stages. Adjacent sections (B,E) were stained for alkaline phosphatase (brown) to reveal the areas containing PGCs (arrows). (C,F) Negative controls using anti-Pin1 antibodies pre-absorbed with Pin1 protein show the specificity of the Pin1 staining. Red in A,C,D,F is DAPI stained nuclei.